| Solution Information | help | |
| Enzyme: | Ion channel NompC | |
| inhibitor: | BDBM46778 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | n/a | |
| Temp. Prep.: | n/a | |
| Comments: | Assay Overview: The purpose of this assay is to determine dose response curves for compounds identified as active in a previous set of experiments entitled, "Primary cell-based high-throughput screening assay to identify agonists of the transient receptor potential channel N1" (PubChem AID 1424), and that were inactive in a previous set of experiments entitled, "Primary cell-based high-throughput screening assay to identify agonists of the transient receptor potential channel ML3" (PubChem AID 1448). This assay employs a HEK293 cell line that stably expresses the zebrafish TRPN1-YFP cation channel. The cells are treated with test compounds followed by measurement of intracellular calcium as monitored by a fluorescent, cell permeable calcium indicator dye. As designed, compounds that act as TRPN1 agonists will increase calcium mobilization, resulting in increased relative fluorescence of the indicator dye, and thus increase well fluorescence. Compounds were tested in triplicate using | |
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